BD CLED Agar (Cystine-Lactose-Electrolyte-Deficient Agar) is a differential culture medium for use in isolating and enumerating bacteria from urine. It supports the growth of urinary pathogens and contaminants but prevents undue swarming of Proteus species due to its lack of electrolytes.
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What are CLED plates used for?
BD CLED Agar (Cystine-Lactose-Electrolyte-Deficient Agar) is a differential culture medium for use in isolating and enumerating bacteria from urine. It supports the growth of urinary pathogens and contaminants but prevents undue swarming of Proteus species due to its lack of electrolytes.
Why do we use CLED to culture urine?
CLED is preferred over a combination of blood agar (BA) and MacConkey agar for routine urine culture because it supports the growth of all potential urinary pathogens, and a number of contaminants such as diphtheroids, lactobacilli, and micrococci.
How do you make CLED agar?
Preparation of CLED Agar Suspend 36 gram of the medium in one liter of distilled water. Mix well and heat with frequent agitation and boil for one minute until complete to dissolution. Autoclave at 121°C for 15 minutes. Cool to 50°C, mix well and dispense into plates.
Is CLED selective or differential?
differential
CLED Agar is a differential culture medium for use in isolating and enumerating bacteria in urine. MacConkey II Agar is a selective and differential medium for the isolation and differentiation of Enterobacteriaceae and a variety of other gram-negative rods from clinical and non-clinical specimens.
Which organism grows on CLED?
Ⅳ. Interpretation of Cled Agar
| Bacteria | Growth |
|---|---|
| Pseudomonas | Green colonies with typical matted surface and rough periphery; blue medium |
| Streptococci | Small, opaque, pale yellow colonies |
| Staphylococci | Very small, opaque, yellow colonies |
| Enterococci | Small yellow colonies, about 0.5 mm in diameter; yellow medium |
What Colour is CLED agar?
Ⅳ. Interpretation of Cled Agar
| Bacteria | Growth |
|---|---|
| Escherichia coli, Citrobacte | Yellow colonies, opaque; yellow medium |
| Enterobacter, Klebsiella | Yellow to whitish-blue colonies, often mucoid; yellowish medium |
| Proteus | Translucent blue colonies; blue-green to blue medium |