What is Waters Acquity UPLC?

Information: The Waters ACQUITY Ultra-Performance Liquid Chromatography (UPLC) System uses a high-resolution liquid chromatography technique for analyzing the molecular components of compounds. Our system is equipped with RI, FLR, and PDA detectors.

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What is H Class UPLC?

The ACQUITY UPLC H-Class is a streamlined system that brings together the flexibility and simplicity of quaternary solvent blending and a flow-through-needle injector to deliver the advanced performance expected of UPLC type separations – high resolution, sensitivity and improved throughput – while maintaining the …

How does a UPLC work?

The underlying principle of UPLC is based on van Deemter statement which describes the connection between linear velocity with plate height. UPLC contributes to the improvement of the three areas: speed, resolution, and sensitivity.

What is H Class HPLC?

Choosing the ACQUITY UPLC H-Class enables you to continue running existing HPLC methods on a forward-looking LC platform that allows you to confidently and seamlessly transition to UPLC separations, when you’re ready, using integrated system tools and reliable column kits for method transfer and method development that …

What is UPLC analysis?

Ultra Performance Liquid Chromatography (UPLC) Based Analysis Services. Ultra Performance Liquid Chromatography (UPLC) is the newest technology in liquid chromatography based analysis. UPLC is the upgrade high performance liquid chromatography with high pressures, outstanding in both peak resolution and sensitivity.

What is a UPLC system?

Ultra-performance liquid chromatography (UPLC) is a combination of a 1.7 μm reverse-phase packing material and a chromatographic system that can operate at pressures in the 6000–15 000 psi range (conventional HPLC uses 3–5 μm packing material and operates between 2000 and 4000 psi).

Why is UPLC used?

UPLC instruments are specially designed to resist higher back-pressures than those supported in conventional LC, providing higher speed, resolution, and sensitivity.

What is the purpose of UPLC?

UPLC is an important method used in the laboratory which reduces the cost and increases the efficiency of analysis required for developing and validating the method. With UPLC, the speed of the separation increases and efficiency improves, which results in the fast development of methodologies.

Is UPLC more expensive than HPLC?

HPLC systems are cheaper than UHPLC systems, and method transfer over to UHPLC is both timely and costly. Time spent transferring methods could be spent developing assays or extracting samples.

How is UPLC different from HPLC?

A: Both HPLC and UPLC are both liquid chromatography techniques used to separate the different components found in mixtures. However, UPLC operates at higher pressures (15,000 psi) and allows for lower particle sizes in columns, while HPLC operates at lower pressures (max <6000 psi).

Is UHPLC and UPLC same?

As vendors entered the market with similar instruments UHPLC was coined as a way to refer to instruments similar to UPLC since UPLC is a Waters trademark. The two terms are understood to be synonymous.